Assistant Engineer, RECONNECT Project
Context
The RECONNECT project focuses on two complementary therapeutic strategies addressing two major roadblocks in optic nerve regenerative medicine: the defective regulation of cytoskeleton dynamics in RGC axons and macrophage-driven inflammation induced by the degenerative process. These strategies are explored by leveraging the unique advantages offered by human retinal organoids derived from pluripotent stem cells, while also integrating additional complementary models to identify and test candidate targets that promote RGC axon growth/regeneration and connectivity.
The position will focus on working with these organoids, including culturing various human iPS cell lines, generating retinal organoids, and purifying and characterizing human RGCs. These cells will constitute high-quality biological material for subsequent transplantation studies in in vitro and in vivo models of RGC degeneration conducted by collaborating members of the consortium. This work will provide hands-on experience with innovative human 3D in vitro models in a cutting-edge translational research environment, while involving the candidate in a collaborative and multidisciplinary consortium. The successful candidate will also participate in the daily organization of lab activities and maintenance of experimental records.
The research team
The Retinal development and repair groupe uses human induced pluripotent stem cells (iPSCs) as a tool to deepen our understanding of human retinal development and its pathologies, as well as to design novel therapeutic strategies, including cell therapy in regenerative medicine, the evaluation of gene therapy approaches, and pharmacological screening.
Missions
We are seeking a motivated assistant engineer to join the RECONNECT project, which focuses on developing innovative regenerative and stem-cell-based therapies for optic neuropathies, particularly glaucoma. Glaucoma is a leading cause of irreversible blindness, resulting from optic nerve damage and progressive degeneration of Retinal Ganglion Cells (RGCs). Despite current treatments, many patients continue to lose vision or are diagnosed too late, highlighting the urgent need for approaches that not only slow disease progression but also restore visual function by repairing or replacing damaged RGCs and by promoting their connections to the brain.
Education and experience
Candidates should hold a Master 2 (or equivalent) in Life Sciences, with strong expertise in molecular and cellular biology. Experience with stem cell culture and/or 3D in vitro models is highly desirable. Strong organizational skills, scientific curiosity, rigor, and ability to work independently as well as collaboratively in multidisciplinary consortia are expected. Working languages are English and French. Salary will follow the University scale, depending on experience.
Specific working conditions
This is a 12-month position, with possibility of extension, offering stimulating and creative working conditions in a dynamic, international, and interdisciplinary research environment.
Applications including a CV, list of publications, a short description of previous research projects, and contact information for at least two referees should be sent by email to gael.orieux@inserm.fr and coralie.fassier@inserm.fr
How to apply
To apply for this vacancy, please complete this form and attach your cover letter and curriculum vitae in .pdf format. We will get back to you as soon as possible.