Développement et régénération de la rétine : apport des cellules souches pluripotentes
Chef d'équipe : Olivier Goureau
INTRODUCTION | EQUIPE | PRESENTATION | DOMAINES DE RECHERCHE | PUBLICATIONS | ILS NOUS SOUTIENNENT
Introduction
Notre équipe développe des approches de thérapie cellulaire utilisant la transplantation de cellules rétiniennes dérivées de cellules souches pluripotentes. Notre objectif est de pouvoir offrir une alternative thérapeutique pour le traitement de maladies dégénératives de la rétine caractérisées par une perte des photorécepteurs et des cellules de l’épithélium pigmentaire rétinien.
Présentation
- Le développement de lignées de cellules iPS humaines porteuses de gènes codant pour des protéines fluorescentes dont l’expression est spécifique de certaines étapes de la différentiation rétinienne ou d’un type cellulaire de la rétine ;
- Le développement de protocoles innovants et robustes permettant la différenciation de cellules iPS humaines en différents types de cellules rétiniennes (cellules ganglionnaires de la rétine, photorécepteurs et cellules de l’épithélium pigmentaire rétinien) ;
- Le développement de cellules iPS issues de donneurs atteints de différentes dystrophies rétiniennes héréditaires. Ces lignées de cellules iPS porteuses de mutations aideront à comprendre les mécanismes pathologiques et permettront également de cribler à grande échelle le potentiel thérapeutique de nouvelles molécules.
Domaines de recherche
- Cellules souches pluripotentes
- Développement de la rétine
- Régénération/réparation de la rétine
- Thérapie cellulaire
Team Leader
Publications
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Liste actualisée et complète par scientifique
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2020
Dynamic full-field optical coherence tomography : 3D live-imaging of retinal organoids
Scholler J, Groux K, Goureau O, Sahel J-A, Fink M, Reichman S, Boccara C and Grieve K. Light Sci. Appl. 2020 Aug 17;9:140.Generation and characterization of transplantable population of human iPSC-derived retinal ganglion cells.Generation and characterization of transplantable population of human iPSC-derived retinal ganglion cells.
Rabesandratana O, Chaffiol A, Mialot A, Slembrouck-Brec A, Joffrois C, Nanteau C, Rodrigues A, Gagliardi G, Reichman S, Sahel JA, Chedotal A, Duebel J, Goureau O and Orieux G. Frontiers Cell Dev. Biol. Oct 27;8:585675.SARS-CoV-2 targets neurons of 3D human brain organoids
Anand Ramani et Müller L, Niklas Ostermann P, Gabriel E, Abida-Islam P, Müller-Schiffmann A, Mariappan A, Goureau O, Gruell H, Walker A, Andrée M, Hauka S, Houwaart T, Dilthey A, Wohlgemuth K, Omran H, Klein F, Wieczorek D, Adams O, Timm J, Korth C, Schaal H, Gopalakrishnan J.
EMBO J (2020)e106230 https://doi.org/10.15252/embj.2020106230Clinical-grade production and safe delivery of human ESC derived RPE sheets in primates and rodents.
Ben M'Barek K, Bertin S, Brazhnikova E, Jaillard C, Habeler W, Plancheron A, Fovet CM, Demilly J, Jarraya M, Bejanariu A, Sahel JA, Peschanski M, Goureau O,CA and Monville C,CA. Biomaterials 2020, 230:119603.AAV-mediated gene delivery to 3D retinal organoids derived from human induced pluripotent stem cells.AAV-mediated gene delivery to 3D retinal organoids derived from human induced pluripotent stem cells.
Garita-Hernandez M, Routet F, Guibbal L, Khabou H, Toualb L, Riancho L, Reichman S, Duebel J, Sahel JA, Goureau O and Dalkara D. Int J Mol Sci. 2020, Feb 3;21(3).Nouvelle approche thérapeutique pour les rétinites pigmentaires : la transplantation de photorécepteurs dérivées de cellules souches.
Goureau O et Orieux G. Médecine/Science 2020 36, 600-606.Les organoïdes de rétine : un nouvel outil pour la compréhension et le traitement des pathologies rétiniennes
Goureau O, Reichman S et Orieux G. Médecine/Science 2020, 36, 626-632. -
2019
Photoreceptor cell replacement in macular degeneration and retinitis pigmentosa: A pluripotent stem cell-based approach.
Gagliardi G, Ben M’Barek K and Goureau O
Prog Retin Eye Res. 2019. 71,1-25.Generation of human induced pluripotent stem cell lines from a patient with ITM2B-related retinal dystrophy and a non-mutated brother
Wohlschlegel J, Letellier C, Liu B, Méjécase , Slembrouck-Brec A2, Condroyer C, Michiels C, Sahel JA, Reichman S, Zeitz C, Goureau O and Audo I
Stem Cell Res. 2019, Nov 5;41:101625.Clinical-grade production and safe delivery of human ESC derived RPE sheets in primates and rodents.
Ben M'Barek K, Bertin S, Brazhnikova E, Jaillard C, Habeler W, Plancheron A, Fovet CM, Demilly J, Jarraya M, Bejanariu A, Sahel JA, Peschanski M, Goureau O and Monville C.
Biomaterials 2019 Nov 6:119603.
Restoration of visual function by transplantation of optogenetically engineered photoreceptors.
Garita-Hernandez M, Lampic M, Chaffiol A, Guibbal L, Routet F, Santos-Ferreira T, Gasparini S, Borsch O, Gagliardi G, Reichman S, Picaud S, Sahel J-A, Goureau O, Ader M, Duebel J and Dalkara D
Nat Comm 2019 Oct 4;10(1):4524.Reprogramming of adult retinal Müller glial cells into human induced pluripotent stem cells as an efficient source of retinal cells
Slembrouck-Brec A, Rodrigues A, Rabesandratana O, Gagliardi G, Nanteau C, Fouquet S, Thuret G, Reichman S, Orieux G and Goureau O
Stem Cell Int. 2019 2019 Jul 15;2019:7858796 -
2018
Characterization and transplantation of CD73-positive photoreceptors isolated from human iPSC-derived retinal organoids.
Gagliardi G, Ben M'Barek K, Chaffiol A, Slembrouck-Brec A, Conart JB, Nanteau C, Rabesandratana O, Sahel J-A, Duebel J, Orieux G, Reichman S, and Goureau O.
Stem Cell Rep. 2018, 11(3):665-680.Defined Xeno-free and Feeder-free culture conditions for the generation of human iPSC-derived retinal cell models.
Slembrouck-Brec A, Nanteau C, Sahel J-A, Goureau O and Reichman S.
J. Vis. Exp. 2018, Sep 6;(139).Engineering transplantation-suitable retinal pigmented epithelium tissue derived from human embryonic stem cells.
Ben M'Barek K, Habeler W, Plancheron A, Jarraya M, Goureau O and Monville C.
J. Vis. Exp. 2018, Sep 6;(139).Pluripotent stem cell-based approaches to explore and treat optic neuropathies.
Rabesandratana O, Goureau O and Orieux G.
rontiers in Neuroscience 2018, Sep 20;12:651.Optogenetic light sensors in human retinal organoids
Garita-Hernandez M, Guibbal L, Toualb L, Routet F, Chaffiol A, Winckler C, Harinquet M, Robert C, Fouquet S, Bellow S, Sahel J-A, Goureau O, Duebel J and Dalkara D.
Frontiers in Neuroscience 2018, Nov 02;12:789.Non-invasive gene delivery to foveal cones for gene therapy and vision restoration.
Khabou H, Garita M, Chaffiol A Reichman S, Jaillard C, Brazhnikova E, Bertin S, Fradot V, Desrosiers M, Winckler C, Goureau O, Picaud S, Duebel J, Sahel J-A, and Dalkara D.
J. Clin. Invest. Insight 2018, Jan 25;3(2) -
2017
Human ESC-derived retinal epithelial cell sheets potentiate rescue of photoreceptor cell loss in rats with retinal degeneration.
Ben M'Barek K, Habeler W, Plancheron A, Jarraya M, Regent F, Terray A, Yang Y, Chatrousse L, Domingues S, Masson Y, Sahel JA, Peschanski M, Goureau O and Monville C.
Sci. Transl Med. 2017, Dec 20;9(421).Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNFα.
Mathis T, Housset M, Eandi C, Beguier F, Touhami S, Reichman S, Augustin S, Gondouin P, Sahel J-A, Kodjikian L, Goureau O, Guillonneau X and Sennlaub F. Aging Cell 2017, 16(1):173-182.Generation of storable retinal organoids and retinal pigmented epithelium from adherent human iPS cells in xeno-free and feeder-free conditions.
Reichman S, Slembrouck A, Gagliardi G, Chaffiol A, Terray A, Nanteau C, Potey A, Belle M, Rabesandratana O, Duebel J, Orieux G, Nandrot EF, Sahel J-A and Goureau O. Stem Cells 2017, 35(5):1176-1188.Generation of an induced pluripotent stem cell line from a patient with autosomal dominant retinitis pigmentosa due to a mutation in the NR2E3 gene.
Terray A, Slembrouck A, Nanteau C, Condroyer C, Zeitz C, Sahel J-A, Audo I, Reichman S, and Goureau O. Stem Cell Research 2017, 24:1-4.Otx2-genetically modified retinal pigment epithelial cells rescue photoreceptors after transplantation.
Kole C, Klipfel L, Yang Y, Ferracane V, Blond F, Reichman S, Millet-Puel G, Clérin E, Aït-Ali N, Pagan D, Camara H, Delyfer MN, Nandrot E, Sahel JA, Goureau O and Léveillard T. Mol. Therapy 2017 [Sep 8. pii: S1525-0016(17)30419-7].Establishment of an induced pluripotent stem (iPS) cell line from dermal fibroblasts of an asymptomatic patient with dominant PRPF31 mutation.
Terray A, Fort V, Slembrouck A, Nanteau C, Sahel J-A, Reichman S, Audo I and Goureau O. Stem Cell Research 2017, 25:26-29.Further insights into the ciliary gene and protein KIZ and its murine ortholog PLK1S1 mutated in rod-cone dystrophy.
El Shamieh S, Méjécasse C, Bertelli M, Terray A, Michiels C, Condroyer C, Fouquet S, Sadoun M, Clérin E, Liu B, Léveillard T, Goureau O, Sahel J-A, Audo I, Zeitz C. Genes 2017, Oct 18; 8(10), 277. -
2016
Let there be light: gene and cell therapy for blindness.
Dalkara D, Goureau O, Marazova K and Sahel J-A.
Hum. Gene Ther. 2016 2,134-147.Gene and cell therapy for inherited retinal dystrophies
Garita-Hernandez M, Goureau O, Dalkara D. (August 2016).
In: eLS. John Wiley & Sons Ltd: Chichester. DOI: 10.1002/9780470015902.a0026565.Production of retinal cells from confluent human iPS cells.
Reichman S and Goureau O.
Methods Mol Biol. 2016 1357:339-351. -
2015
The protein tyrosine phosphatase interacting protein 51 (PTPIP51) is required for the differentiation of photoreceptors.
Orieux G, Slembrouck A, Bensaïd M, Sahel JA and Goureau O.
Neuroscience. 2015, 300, 276-285 -
2014
Production of in vitro retina from pluripotent human stem cells: a new therapeutic tool.
Reichman S, Sahel JA, Goureau O.
Med Sci (Paris). 2014 Oct;30(10):845-8. doi: 10.1051/medsci/20143010009. Epub 2014 Oct 14. French. No abstract available.From confluent human iPS cells to self-forming neural retina and retinal pigmented epithel
Reichman S, Terray A, Slembrouck A, Nanteau C, Orieux G, Habeler W, Nandrot EF, Sahel JA, Monville C, Goureau O.
Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8518-23. doi: 10.1073/pnas.1324212111.El Shamieh S, Neuillé M, Terray A, Orhan E, Condroyer C, Démontant V, Michiels C, Antonio A, Lancelot ME, Letexier M, Saraiva JP, Léveillard T, Mohand-Saïd S, Goureau O, Sahel J-A, Zeitz C, Audo J.
Whole exome sequencing identifies PLK1S1 as a new ciliary gene underlying autosomal recessive rod-cone dystrophy.
Am J Hum Genet. 2014 94(4):625-633.
Orieux G, Picault L, Slembrouck A, Roger JE, Guillonneau X, Sahel J-A, Saule S, McPherson JP, Goureau O. Involvement of Bcl-2-associated transcription factor 1 in the differentiation of early-born retinal cells. J. Neurosci. 2014 34(4): 1530-1541.Audo I, Bujakowska K, Orhan E, El Shamieh S, Sennlaub F, Guillonneau X, Antonio A, Michiels C Lancelot M-E, Letexier M, SaraivaJ-P, Nguyen H, Luu TD, Léveillard T, Poch O, Dollfus H, Paques M, Goureau O, Mohand-Saïd S, Bhattacharya SS, Sahel J-A, Zeitz C. The familial dementia gene revisited: a missense mutation revealed by whole exome sequencing identifies ITM2B as a candidate gene underlying a novel autosomal dominant retinal dystrophy in a large family. Hum Mol Genet. 2014 23(2): 491-501.
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2013
Brevet Européen - N°EP13165654.8 (26/04/2013) Reichman S., Goureau, O., Sahel J-A. METHODS FOR OBTAINING RETINAL PROGENITORS, RETINAL PIGMENTED EPITHELIAL CELLS AND NEURAL RETINAL CELLS.Lustremant C, Habeler W, Plancheron A, Goureau O, Grenot L, de la Grange P, Audo I, Nandrot E, Monville C. Human induced pluripotent stem cells as a tool to model a form of Leber congenital amaurosis.Cell. Reprogramming 2013, 15(3): 233-246.
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2012
Laurent M, Le Dréau G, Guillonneau X, Lelièvre E, Slembrouck A, Goureau O, Martinerie C, Marx M. Temporal and spatial expression of CCN3 during retina development. Dev Neurobiol. 2012, 72(11): 1363–1375.Lelièvre EC, Benayoun BA, Mahieu L, Roger JE, Sahel JA, Sennlaub F, Veitia RA, Goureau O, Guillonneau X. A regulatory domain is required for Foxn4 activity during retinogenesis. J Mol Neurosci.2012, 46(2): 315-32
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