Development, evolution and function on commissural systems

Team leader: Alain Chedotal

Introduction

We explore the role of axon guidance molecules in the regulation of cell-cell interactions during normal development and in pathologies. We also study the function and evolution of commissural connections in the vertebrate central nervous system.

Presentation

Nervous systems are composed of a network of synaptic connections among excitable cells. This network develops as axons extend from presynaptic neurons and grow often long distances to reach their correct postsynaptic partners. Neuronal migration and myelination are also key processes for the formation and stabilization of neuronal connections. For years, neurobiologists have tried to uncover the mechanisms controlling axon guidance. It was found that these are highly conserved between neurons and during evolution. It was also shown that axonal connections are plastic and can be modified during normal physiological processes and in pathological conditions.

From its beginning, our group has studied the development of neuronal connections in the central nervous system (CNS). The field has also gained even more interest in the scientific community when mounting evidence showed that in the nervous system, axon guidance molecules control also other processes (e.g. migration, myelination, apoptosis, plasticity) and that outside the brain they regulate cell communication and cell-cell interaction in many organs during normal development and in pathological conditions such as cancer. Our past and current work aimed at obtaining an extensive view of the role of axon guidance molecules in the vertebrate nervous system. We have mostly studied the function of the Slits and their Roundabout (Robo) receptors and of class 6 transmembrane semaphorins and their Plexin-A receptors.

The aim of our research project is to study extensively the function of axon guidance molecules in the developing and adult central nervous system (CNS). We primarily focus on semaphorins, slits and their receptors and on a few model systems (visual system, cerebellar system, commissural neurons). We try to understand the cellular and molecular mechanisms controlling the development of retinal layers, neural crest cells and brain commissures. We also try to understand how the expression of Robo receptors is regulated. We have generated several conditional knockout lines that are allowing us to start addressing the function of Slit/Robo signaling in postnatal neurogenesis, neo-angiogenesis and cancer. We also study the regulation of cell-cell interactions during myelination and remyelination using novel genetic methods. Last, we try to develop new tools to promote axonal regeneration in the optic nerve and cornea.

Research areas

Development of retinal connectivity and optic nerve regeneration.
Development, function and evolution of brain commissures.
Molecular control of granule cell migration by semaphorins and plexins.
Mechanisms controlling the differentiation and specification of neural crest cells.
Role of axon guidance molecules in angiogenesis and ocular vasoproliferative diseases.
Innervation of the cornea and cornea transplantation.
Regulation of cell-cell interactions during myelination and remyelination by novel genetic methods.

TO DOWNLOAD THE ALUMNI PDF

Publications

Updated and complete list by scientist
Visiting the ORCID links you will find the updated publications list for the following scientists:
- Alain Chedotal [>>]
- Kim Nguyen Ba Charvet [>>]
- Constantino Sotelo [>>]
- Alexandra Rebsam [>>]
- Nacim Bouheraoua [>>]
- Yorick Gitton [>>]
2020

Revisiting the role of Dcc in visual system development with a novel eye clearing method.
Vigouroux RJ, Cesar Q, Chédotal A, Nguyen-Ba-Charvet KT.
Elife. 2020

Tissue clearing and its applications in neuroscience.
Ueda HR, Ertürk A, Chung K, Gradinaru V, Chédotal A, Tomancak P, Keller PJ.
Nat Rev Neurosci. 2020 Feb;21(2):61-7

Neurogenesis and Specification of Retinal Ganglion Cells.
Nguyen-Ba-Charvet KT, Rebsam A.
Int J Mol Sci. 2020 Jan 10;21(2). pii: E451.
2019

Construction and reconstruction of brain circuits: normal and pathological axon guidance.
Roig-Puiggros S, Vigouroux RJ, Beckman D, Bocai NI, Chiou B, Davimes J, Gomez G, Grassi S, Hoque A, Karikari TK, Kiffer F, Lopez M, Lunghi G, Mazengenya P, Meier S, Olguín-Albuerne M, Oliveira MM, Paraíso-Luna J, Pradhan J, Radiske A, Ramos-Hryb AB, Ribeiro MC, Schellino R, Selles MC, Singh S, Theotokis P, Chédotal A.
J Neurochem. 2019

Decoding human fetal liver haematopoiesis.
Popescu DM, Botting RA, Stephenson E, Green K, Webb S, Jardine L, Calderbank EF, Polanski K, Goh I, Efremova M, Acres M, Maunder D, Vegh P, Gitton Y, Park JE, Vento-Tormo R, Miao Z, Dixon D, Rowell R, McDonald D, Fletcher J, Poyner E, Reynolds G, Mather M, Moldovan C, Mamanova L, Greig F, Young MD, Meyer KB, Lisgo S, Bacardit J, Fuller A, Millar B, Innes B, Lindsay S, Stubbington MJT, Kowalczyk MS, Li B, Ashenberg O, Tabaka M, Dionne D, Tickle TL, Slyper M, Rozenblatt-Rosen O, Filby A, Carey P, Villani AC, Roy A, Regev A, Chédotal A, Roberts I, Göttgens B, Behjati S, Laurenti E, Teichmann SA, Haniffa M.
Nature. 2019 Oct;574(7778):365-371

Roles of axon guidance molecules in neuronal wiring in the developing spinal cord.
Chédotal A.
Nat Rev Neurosci. 2019 Jul;20(7):380-396

Shared and differential features of Robo3 expression pattern in amniotes.
Friocourt F, Kozulin P, Belle M, Suárez R, Di-Poï N, Richards LJ, Giacobini P, Chédotal A.
J Comp Neurol. 2019 Aug 15;527(12):2009-2029.

Synergistic Activity of Floor-Plate- and Ventricular-Zone-Derived Netrin-1 in Spinal Cord Commissural Axon Guidance.
Moreno-Bravo JA, Roig Puiggros S, Mehlen P, Chédotal A.
Neuron. 2019 Feb 20;101(4):625-634.e3.

Long-Range Guidance of Spinal Commissural Axons by Netrin1 and Sonic Hedgehog from Midline Floor Plate Cells.
Wu Z, Makihara S, Yam PT, Teo S, Renier N, Balekoglu N, Moreno-Bravo JA, Olsen O, Chédotal A, Charron F, Tessier-Lavigne M.
Neuron. 2019 Feb 20;101(4):635-647.e4.

Genetic Analysis of the Organization, Development, and Plasticity of Corneal Innervation in Mice.
Bouheraoua N, Fouquet S, Marcos-Almaraz MT, Karagogeos D, Laroche L, Chédotal A.
J Neurosci. 2019 Feb 13;39(7):1150-1168.
2018

Commissural neurons transgress the CNS/PNS boundary in absence of ventricular zone-derived netrin 1.
Moreno-Bravo JA, Roig Puiggros S, Blockus H, Dominici C, Zelina P, Mehlen P, Chédotal A.
Development. 2018 Jan 17;145(2).

Non-cell autonomous control of precerebellar neuron migration by Slits and Robos.
Dominici, C., Rappeneau, Q., Zelina, P., Fouquet, S., and Chédotal, A. (2018).
Development 145, dev.150375.
2017

Commissural neurons transgress the CNS/PNS boundary in absence of ventricular zone-derived netrin-1. Moreno-Bravo, J.A., Puiggros, S.R., Blockus, H., Dominici, C., Zelina, P., Mehlen, P., and Chédotal, A. (2017).
Development 145, dev.159400.

Assessment of Confocal Microscopy for the Diagnosis of Polymerase Chain Reaction-Positive Acanthamoeba Keratitis: A Case-Control Study
De Craene S, Knoeri J, Georgeon C, Kestelyn P, Borderie VM.
Ophthalmology. 2017 Sep 28. pii: S0161-6420(17)32297-2. doi: 10.1016/j.ophtha.2017.08.037. [Epub ahead of print]
PubMed PMID: 28965660.

Stromal striae: a new insight into corneal physiology and mechanics.
Grieve K, Ghoubay D, Georgeon C, Latour G, Nahas A, Plamann K, Crotti C,
Bocheux R, Borderie M, Nguyen TM, Andreiuolo F, Schanne-Klein MC, Borderie V.
Sci Rep. 2017 Oct 19;7(1):13584. doi: 10.1038/s41598-017-13194-6. PubMed PMID: 29051516.

Mutations in the netrin-1 gene cause congenital mirror movements.
Méneret A, Franz EA, Trouillard O, Oliver TC, Zagar Y, Robertson SP, Welniarz Q, Gardner RJM, Gallea C, Srour M, Depienne C, Jasoni CL, Dubacq C, Riant F, Lamy JC, Morel MP, Guérois R, Andreani J, Fouquet C, Doulazmi M, Vidailhet M, Rouleau GA, Brice A, Chédotal A, Dusart I, Roze E, Markie D.
J Clin Invest. 2017 Sep 25. pii: 95442.

Neuroscience in the third dimension: shedding new light on the brain with tissue clearing.
Vigouroux RJ, Belle M, Chédotal A.
Mol Brain. 2017 Jul 20;10(1):33

Behavioral Consequences of a Bifacial Map in the Mouse Somatosensory Cortex.
Tsytsarev V, Arakawa H, Zhao S, Chédotal A, Erzurumlu RS.
J Neurosci. 2017 Jul 26;37(30):7209-7218.

Floor-plate-derived netrin-1 is dispensable for commissural axon guidance.
Dominici C, Moreno-Bravo JA, Puiggros SR, Rappeneau Q, Rama N, Vieugue P, Bernet A, Mehlen P, Chédotal A.
Nature. 2017 May 18;545(7654):350-354.

A mutant with bilateral whisker to barrel inputs unveils somatosensory mapping rules in the cerebral cortex.
Renier N, Dominici C, Erzurumlu RS, Kratochwil CF, Rijli FM, Gaspar P, Chédotal A.
Elife. 2017 Mar 28;6. pii: e23494.

Non cell-autonomous role of DCC in the guidance of the corticospinal tract at the midline.
Welniarz Q, Morel MP, Pourchet O, Gallea C, Lamy JC, Cincotta M, Doulazmi M, Belle M, Méneret A, Trouillard O, Ruiz M, Brochard V, Meunier S, Trembleau A, Vidailhet M, Chédotal A, Dusart I, Roze E.
Sci Rep. 2017 Mar 24;7(1):410.

The cytoskeleton-associated protein SCHIP1 is involved in axon guidance, and is required for piriform cortex and anterior commissure development.
Klingler E, Martin PM, Garcia M, Moreau-Fauvarque C, Falk J, Chareyre F, Giovannini M, Chédotal A, Girault JA, Goutebroze L.
Development. 2015 Jun 1;142(11):2026-36.

Nkx2.1-derived astrocytes and neurons together with Slit2 are indispensable for anterior commissure formation.
Minocha S, Valloton D, Ypsilanti AR, Fiumelli H, Allen EA, Yanagawa Y, Marin O, Chédotal A, Hornung JP, Lebrand C.
Nat Commun. 2015 Apr 23;6:6887.

Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
Rama N, Dubrac A, Mathivet T, Ní Chárthaigh RA, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chédotal A.
Nat Med. 2015 May;21(5):483-91.

Tridimensional visualization and analysis of early human development
Morgane Belle, David Godefroy, Gérard Couly, Samuel A. Malone, Francis Collier, Paolo Giacobini and Alain Chédotal
Cell, 23 March 2017 DOI: 10.1016/j.cell.2017.03.008
2016

Imaging Microscopic Features of Keratoconic Corneal Morphology.
Grieve, K., Georgeon, C., Andreiuolo, F., Borderie, M., Ghoubay, D., Rault, J., and Borderie, V.M. (2016a). Cornea.

Appearance of the Retina With Full-Field Optical Coherence Tomography.
Grieve, K., Thouvenin, O., Sengupta, A., Borderie, V.M., and Paques, M. (2016b). Invest Ophthalmol Vis Sci 57, OCT96-OCT104.

Reverse Signaling by Semaphorin-6A Regulates Cellular Aggregation and Neuronal Morphology.
Perez-Branguli, F., Zagar, Y., Shanley, D.K., Graef, I.A., Chédotal, A., and Mitchell, K.J. (2016). PLoS One 11, e0158686.

A Secreted Slit2 Fragment Regulates Adipose Tissue Thermogenesis and Metabolic Function.
Svensson, K.J., Long, J.Z., Jedrychowski, M.P., Cohen, P., Lo, J.C., Serag, S., Kir, S., Shinoda, K., Tartaglia, J.A., Rao, R.R., Chédotal, A., Kajimura, S., Gygi, S.P., and Spiegelman, B.M. (2016). Cell Metab 23, 454-466.

Development of the neurons controlling fertility in humans: new insights from 3D imaging and transparent fetal brains.
Casoni, F., Malone, S.A., Belle, M., Luzzati, F., Collier, F., Allet, C., Hrabovszky, E., Sowmyalakshmi, R., Prévot, V., Chédotal, A. and Giacobini, P. (2016) Development, 143, 3969-3981.

PlexinA2 and Sema6A are required for retinal progenitor cell migration.
Belle, M., Parray, A., Belle, M., Chédotal, A., and Nguyen-Ba-Charvet, K.T. (2016). Dev Growth Differ 58, 492-502.

Incidence of Intraocular Pressure Elevation and Glaucoma after Lamellar versus Full-Thickness Penetrating Keratoplasty.
Borderie, V.M., Loriaut, P., Bouheraoua, N., and Nordmann, J.P. (2016). Ophthalmology 123, 1428-1434.

New parameters in assessment of human donor corneal stroma.
Borderie M, Grieve K., Irsch K., Ghoubay D., Georgeon C., De Sousa C., Laroche L, Borderie VM. (2016) Acta Ophthalmologica, 95:297-306.

Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization.
Dubrac, A., Genet, G., Ola, R., Zhang, F., Pibouin-Fragner, L., Han, J., Zhang, J., Thomas, J.L., Chédotal, A., Schwartz, M.A., and Eichmann, A. (2016). Circulation 133, 409-421.

The Pluripotency of Neural Crest Cells and Their Role in Brain Development.
Le Douarin NM, Dupin E.
In "Essays on Developmental Biology Part A", Current Topics in Developmental Biology, vol 116. 2016 in press (available online 20 January). doi:10.1016/bs.ctdb.2015.10.008.

Slit-Robo signaling.
Blockus, H., and Chédotal, A. Development 143, 3037-3044, 2016a.

Fly Dscams Can Also Help You Find the Right Partners.
Blockus, H., and Chédotal, A. Neuron 89, 423-425, 2016b.
2015

Endothelial Survival After Descemet Membrane Endothelial Keratoplasty: Effect of Surgical Indication and Graft Adherence Status.
Baydoun, L., Ham, L., Borderie, V., Dapena, I., Hou, J., Frank, L.E., Oellerich, S., and Melles, G.R. (2015). JAMA Ophthalmol 133, 1277-1285.

Iris-fixated phakic intraocular lens implantation to correct myopia and a predictive model of endothelial cell loss.
Bouheraoua, N., Bonnet, C., Labbe, A., Sandali, O., Lecuen, N., Ameline, B., Borderie, V., and Laroche, L. (2015a). J Cataract Refract Surg 41, 2450-2457.

Decreased Corneal Sensation and Subbasal Nerve Density, and Thinned Corneal Epithelium as a Result of 360-Degree Laser Retinopexy.
Bouheraoua, N., Hrarat, L., Parsa, C.F., Akesbi, J., Sandali, O., Goemaere, I., Hamiche, T., Laroche, L., and Borderie, V. (2015b). Ophthalmology 122, 2095-2102.

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis. Bouheraoua, N., Jouve, L., Borderie, V., and Laroche, L. (2015c). J Vis Exp.

PlexinA1 is a new Slit receptor and mediates axon guidance function of Slit C-terminal fragments.
Delloye-Bourgeois, C., Jacquier, A., Charoy, C., Reynaud, F., Nawabi, H., Thoinet, K., Kindbeiter, K., Yoshida, Y., Zagar, Y., Kong, Y., Jones, Y.E., Falk, J., Chédotal, A., and Castellani, V. (2015). Nature Neurosci 18, 36-45.

Multicolor analysis of oligodendrocyte morphology, interactions, and development with Brainbow.
Dumas, L., Heitz-Marchaland, C., Fouquet, S., Suter, U., Livet, J., Moreau-Fauvarque, C., and Chédotal, A. (2015). Glia 63, 699-717.

Fourier-domain optical coherence tomography imaging in corneal epithelial basement membrane dystrophy: a structural analysis.
El Sanharawi, M., Sandali, O., Basli, E., Bouheraoua, N., Ameline, B., Goemaere, I., Georgeon, C., Hamiche, T., Borderie, V., and Laroche, L. (2015). Am J Ophthalmol 159, 755-763.

Anatomic Predictive Factors of Acute Corneal Hydrops in Keratoconus: An Optical Coherence Tomography Study.
Fuentes, E., Sandali, O., El Sanharawi, M., Basli, E., Hamiche, T., Goemaere, I., Borderie, V., Bouheraoua, N., and Laroche, L. (2015). Ophthalmology 122, 1653-1659.

Full-field optical coherence tomography of human donor and pathological corneas.
Ghouali, W., Grieve, K., Bellefqih, S., Sandali, O., Harms, F., Laroche, L., Paques, M., and Borderie, V. (2015). Curr Eye Res 40, 526-534.

Three-dimensional structure of the mammalian limbal stem cell niche.
Grieve, K., Ghoubay, D., Georgeon, C., Thouvenin, O., Bouheraoua, N., Paques, M., and Borderie, V.M. (2015). Exp Eye Res 140, 75-84.

The cytoskeleton-associated protein SCHIP1 is involved in axon guidance, and is required for piriform cortex and anterior commissure development.
Klingler, E., Martin, P.M., Garcia, M., Moreau-Fauvarque, C., Falk, J., Chareyre, F., Giovannini, M., Chédotal, A., Girault, J.A., and Goutebroze, L. (2015). Development 142, 2026-2036.

Imported pythium insidiosum keratitis after a swim in Thailand by a contact lens-wearing traveler.
Lelievre, L., Borderie, V., Garcia-Hermoso, D., Brignier, A.C., Sterkers, M., Chaumeil, C., Lortholary, O., and Lanternier, F. (2015). Am J Trop Med Hyg 92, 270-273.

Femtosecond-Assisted Arcuate Keratotomy for the Correction of Postkeratoplasty Astigmatism: Vector Analysis and Accuracy of Laser Incisions.
Loriaut, P., Borderie, V.M., and Laroche, L. (2015a). Cornea 34, 1063-1066.

Deep sclerectomy versus trabeculectomy in eyes with penetrating keratoplasty.
Loriaut, P., Nordmann, J.P., Laroche, L., and Borderie, V.M. (2015b). Cornea 34, 375-380.

Long-term outcomes after mechanized arcuate keratotomy for the correction of astigmatism after keratoplasty.
Loriaut, P., Tumahai, P., Hoffart, L., Borderie, V.M., and Laroche, L. (2015c). J Refract Surg 31, 71-72.

Nkx2.1-derived astrocytes and neurons together with Slit2 are indispensable for anterior commissure formation.
Minocha, S., Valloton, D., Ypsilanti, A.R., Fiumelli, H., Allen, E.A., Yanagawa, Y., Marin, O., Chédotal, A., Hornung, J.P., and Lebrand, C. (2015). Nature Commun 6, 6887.

Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
Rama, N., Dubrac, A., Mathivet, T., Ni Charthaigh, R.A., Genet, G., Cristofaro, B., Pibouin-Fragner, L., Ma, L., Eichmann, A., and Chédotal, A. (2015). Nature Medicine 21, 483-491.

Corneal epithelial thickness mapping using Fourier-domain optical coherence tomography for detection of form fruste keratoconus.
Temstet, C., Sandali, O., Bouheraoua, N., Hamiche, T., Galan, A., El Sanharawi, M., Basli, E., Laroche, L., and Borderie, V. (2015). J Cataract Refract Surg 41, 812-820.

Development of human corneal epithelium on organized fibrillated transparent collagen matrices synthesized at high concentration.
Tidu, A., Ghoubay-Benallaoua, D., Lynch, B., Haye, B., Illoul, C., Allain, J.M., Borderie, V.M., and Mosser, G. (2015). Acta Biomater 22, 50-58.
2014

Signaling switch of the axon guidance receptor Robo3 during vertebrate evolution.
Zelina P, Blockus H, Zagar Y, Péres A, Friocourt F, Wu Z, Rama N, Fouquet C, Hohenester E, Tessier-Lavigne M, Schweitzer J, Roest Crollius H, Chédotal A.
Neuron. 2014 Dec 17;84(6):1258-72. doi: 10.1016/j.neuron.2014.11.004. Epub 2014 Nov 26.
PMID:25433640

Development and plasticity of commissural circuits: from locomotion to brain repair.
Chédotal A.
Trends Neurosci. 2014 Oct;37(10):551-62. doi: 10.1016/j.tins.2014.08.009. Epub 2014 Sep 11. Review.
PMID:25220044

Sonic Hedgehog promotes the survival of neural crest cells by limiting apoptosis induced by the dependence receptor CDON during branchial arch. development.
Delloye-Bourgeois C, Rama N, Brito J, Le Douarin N, Mehlen P.
Biochem Biophys Res Commun. 2014 Sep 26;452(3):655-60. doi: 10.1016/j.bbrc.2014.08.134.1.
PubMed PMID: 25193697.

The neural crest, a multifaceted structure of the vertebrates.
Dupin E, Le Douarin NM.
Birth Defects Res C Embryo Today. 2014 Sep;102(3):187-209. doi:
10.1002/bdrc.21080. PubMed PMID: 25219958.

Combinatorial activity of Six1-2-4 genes in cephalic neural crest cells controls craniofacial and brain development.
Garcez RC, Le Douarin NM, Creuzet SE.
Cell Mol Life Sci. 2014 Jun;71(11):2149-64. doi: 10.1007/s00018-013-1477-z. PubMed PMID: 24061537.

The multifaceted roles of Slits and Robos in cortical circuits: from proliferation to axon guidance and neurological diseases.
Blockus H, Chédotal A.
Curr Opin Neurobiol. 2014 Aug;27:82-8. doi: 10.1016/j.conb.2014.03.003. Epub 2014 Mar 31. Review.
PMID: 24698714

Multicolor analysis of oligodendrocyte morphology, interactions, and development with Brainbow.
Dumas L, Heitz-Marchaland C, Fouquet S, Suter U, Livet J, Moreau-Fauvarque C, Chédotal A.Glia. 2014 Dec 21. doi: 10.1002/glia.22779. [Epub ahead of print]

PlexinA1 is a new Slit receptor and mediates axon guidance function of Slit C-terminal fragments.
Delloye-Bourgeois C, Jacquier A, Charoy C, Reynaud F, Nawabi H, Thoinet K, Kindbeiter K, Yoshida Y, Zagar Y, Kong Y, Jones YE, Falk J, Chédotal A, Castellani V.
Nat Neurosci. 2014 Dec 8. doi: 10.1038/nn.3893. [Epub ahead of print]

A Simple Method for 3D Analysis of Immunolabeled Axonal Tracts in a Transparent Nervous System.
Belle M, Godefroy D, Dominici C, Heitz-Marchaland C, Zelina P, Hellal F, Bradke F, Chédotal A.
Cell Rep. 2014 Nov 20;9(4):1191-201. doi: 10.1016/j.celrep.2014.10.037. Epub 2014 Nov 6.

Signaling Switch of the Axon Guidance Receptor Robo3 during Vertebrate Evolution.
Zelina P, Blockus H, Zagar Y, Péres A, Friocourt F, Wu Z, Rama N, Fouquet C, Hohenester E, Tessier-Lavigne M, Schweitzer J, Roest Crollius H, Chédotal A.
Neuron. 2014 Dec 17;84(6):1258-72. doi: 10.1016/j.neuron.2014.11.004. Epub 2014 Nov 26.

Time-lapse analysis of tangential migration in Sema6A and PlexinA2 knockouts.
Renaud J, Chédotal A.
Mol Cell Neurosci. 2014 Nov;63:49-59. doi: 10.1016/j.mcn.2014.09.005. Epub 2014 Oct 3.

Development and plasticity of commissural circuits: from locomotion to brain repair.
Chédotal A.
Trends Neurosci. 2014 Oct;37(10):551-62. doi: 10.1016/j.tins.2014.08.009. Epub 2014 Sep 11. Review.

Development of retinal layers.
Nguyen-Ba-Charvet KT, Chédotal A.
C R Biol. 2014 Mar;337(3):153-9. doi: 10.1016/j.crvi.2013.11.010. Epub 2014 Mar 5. Review.

The multifaceted roles of Slits and Robos in cortical circuits: from proliferation to axon guidance and neurological diseases.
Blockus H, Chédotal A.
Curr Opin Neurobiol. 2014 Aug;27:82-8. doi: 10.1016/j.conb.2014.03.003. Epub 2014 Mar 31.

Dendrite self-avoidance requires cell-autonomous slit/robo signaling in cerebellar purkinje cells.
Gibson DA, Tymanskyj S, Yuan RC, Leung HC, Lefebvre JL, Sanes JR, Chédotal A, Ma L.
Neuron. 2014 Mar 5;81(5):1040-56. doi: 10.1016/j.neuron.2014.01.009

Multiplex cell and lineage tracking with combinatorial labels.
Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire E, Morin X, Livet J.
Neuron. 2014 Feb 5;81(3):505-20. doi: 10.1016/j.neuron.2013.12.016.

Imported Pythium insidiosum Keratitis After a Swim in Thailand by a Contact Lens-Wearing Traveler.
Lelievre L, Borderie V, Garcia-Hermoso D, Brignier AC, Sterkers M, Chaumeil C, Lortholary O, Lanternier F.
Am J Trop Med Hyg. 2014 Dec 22. pii: 14-0380. [Epub ahead of print]

Optical coherence tomography and confocal microscopy following three different protocols of corneal collagen-crosslinking in keratoconus.
Bouheraoua N, Jouve L, El Sanharawi M, Sandali O, Temstet C, Loriaut P, Basli E, Borderie V, Laroche L.
Invest Ophthalmol Vis Sci. 2014 Oct 28;55(11):7601-9. doi: 10.1167/iovs.14-15662

Influence of surgical technique on graft and endothelial survival in endothelial keratoplasty.
Borderie VM, Georgeon C, Bouheraoua N.
J Fr Ophtalmol. 2014 Nov;37(9):675-81. doi: 10.1016/j.jfo.2014.05.006. Epub 2014 Oct 5. French.

Full-Field Optical Coherence Tomography of Human Donor and Pathological Corneas.
Ghouali W, Grieve K, Bellefqih S, Sandali O, Harms F, Laroche L, Paques M, Borderie V.
Curr Eye Res. 2014 Sep 24:1-9. [Epub ahead of print]

Radial keratoneuritis imaging in a patient with Acanthamoeba keratitis
Bouheraoua N, Sandali O, Laroche L, Borderie V.
J Fr Ophtalmol. 2014 Nov;37(9):752-3. doi: 10.1016/j.jfo.2014.04.005. Epub 2014 Sep 8. French. No abstract available.

Acanthamoeba keratitis
Bouheraoua N, Labbé A, Chaumeil C, Liang Q, Laroche L, Borderie V.
J Fr Ophtalmol. 2014 Oct;37(8):640-52. doi: 10.1016/j.jfo.2014.05.004. Epub 2014 Aug 29. French.

New combined technique of deep intrastromal arcuate keratotomy overlayed by LASIK flap for treatment of high astigmatism.
Loriaut P, Sandali O, El Sanharawi M, Goemaere I, Borderie V, Laroche L.
Cornea. 2014 Oct;33(10):1123-8. doi: 10.1097/ICO.0000000000000236.

Cornea in high axial myopia.
Touzeau O, Gaujoux T, Sandali O, Allouch C, Laroche L, Borderie V.
J Fr Ophtalmol. 2014 Jun;37(6):449-61. doi: 10.1016/j.jfo.2014.01.008. Epub 2014 May 27. French.

In Vivo 3-dimensional corneal epithelial thickness mapping as an indicator of dry eye: preliminary clinical assessment.
El Sanharawi M, Sandali O, Borderie V, Laroche L.
Am J Ophthalmol. 2014 May;157(5):1116. doi: 10.1016/j.ajo.2014.01.029. No abstract available.

A reproducible automated segmentation algorithm for corneal epithelium cell images from in vivo laser scanning confocal microscopy.
Bullet J, Gaujoux T, Borderie V, Bloch I, Laroche L.
Acta Ophthalmol. 2014 Jun;92(4):e312-6. doi: 10.1111/aos.12304. Epub 2014 Mar 26.

Bilateral bacterial keratitis following photorefractive keratectomy
Ghouali W, Sandali O, Basli E, Laroche L, Borderie V.
J Fr Ophtalmol. 2014 Mar;37(3):e39-41. doi: 10.1016/j.jfo.2013.05.029. Epub 2014 Feb 26. French. No abstract available.

Corneal radius of curvature after anterior lamellar versus penetrating keratoplasty.
Borderie VM, Georgeon C, Borderie M, Bouheraoua N, Touzeau O, Laroche L.
Graefes Arch Clin Exp Ophthalmol. 2014 Mar;252(3):449-56. doi: 10.1007/s00417-013-2545-7. Epub 2013 Dec 14.

High-resolution spectral domain OCT images of a corneal epithelial inclusion cyst after amniotic membrane transplantation
Qu L, Laroche L, Borderie V.
J Fr Ophtalmol. 2014 Feb;37(2):181-2. doi: 10.1016/j.jfo.2013.05.019. Epub 2013 Nov 6. French. No abstract available.

Increased reaction after cross-linking in keratoconus melanoderm patients.
Sandali O, Ghouali W, Basli E, Bouheraoua N, Goemaere I, Borderie V, Laroche L.
Ocul Immunol Inflamm. 2014 Aug;22(4):333-5. doi: 10.3109/09273948.2013.845229. Epub 2013 Nov 8. No abstract available.

Structural imaging of keratoconus after corneal cross-linking.
Basli E, Sandali O, Touzeau O, Goemaere I, Borderie V, Laroche L.
J Fr Ophtalmol. 2014 Jan;37(1):82-4. doi: 10.1016/j.jfo.2013.05.005. Epub 2013 Aug 9. French. No abstract available.
2013

Badura, A., Schonewille, M., Voges, K., Galliano, E., Renier, N., Gao, Z., Witter, L., Hoebeek, F.E., Chédotal, A., and De Zeeuw, C.I. (2013). Climbing fiber input shapes reciprocity of Purkinje cell firing. Neuron 78, 700-713.

Borderie, V.M., Sandali, O., Basli, E., Goldschmidt, P., and Laroche, L. (2013). Donor tissue selection for anterior lamellar keratoplasty. Cornea 32, 1105-1109.

Bouheraoua, N., Gaujoux, T., Goldschmidt, P., Chaumeil, C., Laroche, L., and Borderie, V.M. (2013). Prognostic factors associated with the need for surgical treatments in acanthamoeba keratitis. Cornea 32, 130-136.

Chen, X.R., Heck, N., Lohof, A.M., Rochefort, C., Morel, M.P., Wehrle, R., Doulazmi, M., Marty, S., Cannaya, V., Avci, H.X., Mariani, J., Rondi-Reig, L., Vodjdani, G., Sherrard, R.M., Sotelo, C., and Dusart, I. (2013). Mature Purkinje cells require the retinoic acid-related orphan receptor-alpha (RORalpha) to maintain climbing fiber mono-innervation and other adult characteristics. J Neurosci 33, 9546-9562.

Coelho-Aguiar, J.M., Le Douarin, N. and Dupin, E. (2013). Environmental factors unveil dormant developmental capacities in multipotent progenitors of the trunk neural crest. Dev Biol 384(1):13-25.

Ghoubay-Benallaoua, D., Sandali, O., Goldschmidt, P., and Borderie, V. (2013). Kinetics of expansion of human limbal epithelial progenitor cells in primary culture of explants without feeders. PLoS One 8, e81965.

Guilbert, E., Bullet, J., Sandali, O., Basli, E., Laroche, L., and Borderie, V.M. (2013). Long-term rejection incidence and reversibility after penetrating and lamellar keratoplasty. Am J Ophthalmol 155, 560-569 e562.

Ichim G, Genevois AL, Ménard M, Yu LY, Coelho-Aguiar JM, Llambi F, Jarrosson-Wuilleme L, Lefebvre J, Tulasne D, Dupin E, Le Douarin N, Arumäe U, Tauszig-Delamasure S, Mehlen P. (2013). The dependence receptor TrkC triggers mitochondria-dependent apoptosis upon Cobra-1 recruitment. Mol Cell 12;51(5):632-46.

Lebrun, C., Avci, H.X., Wehrle, R., Doulazmi, M., Jaudon, F., Morel, M.P., Rivals, I., Ema, M., Schmidt, S., Sotelo, C., Vodjdani, G., and Dusart, I. (2013). Klf9 is necessary and sufficient for Purkinje cell survival in organotypic culture. Mol Cell Neurosci 54, 9-21.

Michalski, N., Babai, N., Renier, N., Perkel, D.J., Chédotal, A., and Schneggenburger, R. (2013). Robo3-driven axon midline crossing conditions functional maturation of a large commissural synapse. Neuron 78, 855-868.

Mommersteeg, M.T., Andrews, W.D., Ypsilanti, A.R., Zelina, P., Yeh, M.L., Norden, J., Kispert, A., Chédotal, A., Christoffels, V.M., and Parnavelas, J.G. (2013). Slit-roundabout signaling regulates the development of the cardiac systemic venous return and pericardium. Circ Res 112, 465-475.

Sandali, O., El Sanharawi, M., Basli, E., Bonnel, S., Lecuen, N., Barale, P.O., Borderie, V., Laroche, L., and Monin, C. (2013a). Epiretinal membrane recurrence: incidence, characteristics, evolution, and preventive and risk factors. Retina 33, 2032-2038.

Sandali, O., El Sanharawi, M., Temstet, C., Hamiche, T., Galan, A., Ghouali, W., Goemaere, I., Basli, E., Borderie, V., and Laroche, L. (2013b). Fourier-domain optical coherence tomography imaging in keratoconus: a corneal structural classification. Ophthalmology 120, 2403-2412.

Troke, P., Obenga, G., Gaujoux, T., Goldschmidt, P., Bienvenu, A.L., Cornet, M., Grenouillet, F., Pons, D., Ranque, S., Sitbon, K., Chaumeil, C., Borderie, V., and Lortholary, O. (2013). The efficacy of voriconazole in 24 ocular Fusarium infections. Infection 41, 15-20.
2012

Whole-exome sequencing identifies mutations in GPR179 leading to autosomal-recessive complete congenital stationary night blindness.
Audo, I., Bujakowska, K., Orhan, E., Poloschek, C.M., Defoort-Dhellemmes, S., Drumare, I., Kohl, S., Luu, T.D., Lecompte, O., Zrenner, E., Lancelot, M.E., Antonio, A., Germain, A., Michiels, C., Audier, C., Letexier, M., Saraiva, J.P., Leroy, B.P., Munier, F.L., Mohand-Said, S., Lorenz, B., Friedburg, C., Preising, M., Kellner, U., Renner, A.B., Moskova-Doumanova, V., Berger, W., Wissinger, B., Hamel, C.P., Schorderet, D.F., De Baere, E., Sharon, D., Banin, E., Jacobson, S.G., Bonneau, D., Zanlonghi, X., Le Meur, G., Casteels, I., Koenekoop, R., Long, V.W., Meire, F., Prescott, K., de Ravel, T., Simmons, I., Nguyen, H., Dollfus, H., Poch, O., Leveillard, T., Nguyen-Ba-Charvet, K., Sahel, J.A., Bhattacharya, S.S., and Zeitz, C. (2012). Am J Hum Genet 90, 321-330.

Thyroid hormone triggers the developmental loss of axonal regenerative capacity via thyroid hormone receptor alpha1 and kruppel-like factor 9 in Purkinje cells.
Avci, H.X., Lebrun, C., Wehrle, R., Doulazmi, M., Chatonnet, F., Morel, M.P., Ema, M., Vodjdani, G., Sotelo, C., Flamant, F., and Dusart, I. (2012). Proc Natl Acad Sci U S A 109, 14206-14211.

Role of transmembrane semaphorin Sema6A in oligodendrocyte differentiation and myelination.
Bernard, F., Moreau-Fauvarque, C., Heitz-Marchaland, C., Zagar, Y., Dumas, L., Fouquet, S., Lee, X., Shao, Z., Mi, S., and Chédotal, A. (2012). Glia 60, 1590-1604.

Association of anti-herpes simplex virus IgG in tears and serum with clinical presentation in patients with presumed herpetic simplex keratitis.
Borderie, V.M., Gineys, R., Goldschmidt, P., Batellier, L., Laroche, L., and Chaumeil, C. (2012a). Cornea 31, 1251-1256.

Long-term results of deep anterior lamellar versus penetrating keratoplasty.
Borderie, V.M., Sandali, O., Bullet, J., Gaujoux, T., Touzeau, O., and Laroche, L. (2012b). Ophthalmology 119, 249-255.

Purkinje cell maturation participates in the control of oligodendrocyte differentiation: role of sonic hedgehog and vitronectin.
Bouslama-Oueghlani, L., Wehrle, R., Doulazmi, M., Chen, X.R., Jaudon, F., Lemaigre-Dubreuil, Y., Rivals, I., Sotelo, C., and Dusart, I. (2012). PLoS One 7, e49015.

Slit2 and Robo3 modulate the migration of GnRH-secreting neurons. Cariboni, A., Andrews, W.D., Memi, F., Ypsilanti, A.R., Zelina, P., Chédotal, A., and Parnavelas, J.G. (2012). Development 139, 3326-3331.

Effects of isoproterenol and cholera toxin on human limbal epithelial cell cultures.
Ghoubay-Benallaoua, D., Pecha, F., Goldschmidt, P., Fialaire-Legendre, A., Chaumeil, C., Laroche, L., and Borderie, V.M. (2012). Curr Eye Res 37, 644-653.

New strategy for rapid diagnosis and characterization of keratomycosis.
Goldschmidt, P., Degorge, S., Benallaoua, D., Semoun, O., Borsali, E., Le Bouter, A., Batellier, L., Borderie, V., Laroche, L., and Chaumeil, C. (2012a). Ophthalmology 119, 945-950.

New strategy for rapid diagnosis and characterization of fungal infections: the example of corneal scrapings.
Goldschmidt, P., Degorge, S., Che Sarria, P., Benallaoua, D., Semoun, O., Borderie, V., Laroche, L., and Chaumeil, C. (2012b). PLoS One 7, e37660.

Guidance-cue control of horizontal cell morphology, lamination, and synapse formation in the mammalian outer retina.
Matsuoka, R.L., Jiang, Z., Samuels, I.S., Nguyen-Ba-Charvet, K.T., Sun, L.O., Peachey, N.S., Chédotal, A., Yau, K.W., and Kolodkin, A.L. (2012). J Neurosci 32, 6859-6868.

Modulation of Bmp4 signalling in the epithelial-mesenchymal interactions that take place in early thymus and parathyroid development in avian embryos.
Neves, H., Dupin, E., Parreira, L., and Le Douarin, N.M. (2012). Dev Biol 361, 208-219.

Plexin-B2 regulates the proliferation and migration of neuroblasts in the postnatal and adult subventricular zone.
Saha, B., Ypsilanti, A.R., Boutin, C., Cremer, H., and Chédotal, A. (2012). J Neurosci 32, 16892-16905.

Paracentral retinal holes occurring after macular surgery: incidence, clinical features, and evolution.
Sandali, O., El Sanharawi, M., Basli, E., Lecuen, N., Bonnel, S., Borderie, V., Laroche, L., and Monin, C. (2012b). Graefes Arch Clin Exp Ophthalmol 250, 1137-1142.

Infectious keratitis in severe limbal stem cell deficiency: characteristics and risk factors.
Sandali, O., Gaujoux, T., Goldschmidt, P., Ghoubay-Benallaoua, D., Laroche, L., and Borderie, V.M. (2012c). Ocul Immunol Inflamm 20, 182-189.