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This course intends to bring together researchers interested on working on optical brain manipulation with the aim to help the attendees in choosing the most suitable combination of actuator, illumination method and targeting strategy for each specific application and learn the skills and technologies required to design and build up an optical system for optical control of neuronal circuits.

Pre-registrations are opened, first selection of candidates will be done by June 25th. A preregistration is required together with the filling of a survey form. Selected candidates will be informed by July 1st at the latest.

The complete program (to dowload here as PDF), other details, and registration information can be found at:
https://cnrsformation.cnrs.fr/stage-19146-Optical-control-of-brain-functioning-with-optogenetics-and-wave-front-shaping.html

OBJECTIVES

*To be able to choose the most suitable combination of actuator, illumination methods and targeting strategy for each specific application

*To acquire the skills and technologies required to design and build up an optical system for optical control of neuronal circuits

AUDIENCE
Neurophysiologists, physicists (researchers, phD students or engineers)

PRE-REQUIREMENT
Attendees should have a strong background in neurosciences, in cell biology or in optical microscopy. To adapt the programme to trainees’ expectations, we invite the attendees to download and fill out the survey from the CNRS web site.

TRAINING PROGRAM
Lectures
- Introduction to optogenetics
- Opsin photo-cycle modeling
- Wave front shaping and liquid crystal matrix
- Computer generated holography
- Generalized phase contrast
- Temporal focusing
- Three dimensional light patterning and temporal focusing
- Two-photon optogenetics: scanning, spiral scanning, parallel illumination: examples and comparison among the different approaches
- How to build up an holographic optical set-up
- All-optical interrogation of brain circuits
- Patterned voltage and calcium imaging
- Viral vector design
- In depth patterned optogenetics

Practical courses (in small rotating groups of 7 participants max with 2 trainers by group)
- How to build up an holographic optical set up (optical design and software)
- In vitro and in vivo all-optical manipulation of neuronal circuits
- Projects from applicants (practical): proposition of projects by the participants and discussion on their feasibility

SPEAKERS
V. Emiliani, E. Papagiakoumou, N. Accanto, E. Ronzitti, D. Dalkara, D. Tanese (researchers, IDV, Paris), C. Wyart (researcher, ICM, Paris), D. Oron, O. Yizhar (researchers, Weizmann Institute) and P. Hagemman (PR, Institut for experimental biophysics, Berlin)

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Useful Information (flyer with programm to download)

Date : Monday 7th October - Friday 11th October 2019
Deadline for pre-registration : 25th June 2019

Places available are limited: book fast!
For information and registration contact : eirini.papagiakoumou@inserm.fr