DONATE MAINS

photo2The photobiology platform was created to investigate visible light toxicity on retinal cells and to evaluate the protection performance of filtering devices.

It aims at defining precisely the most phototoxic wavelengths in the blue section of the visible light spectrum with a sunlight physiological exposure of retinal cells. For that purpose, a cellular illumination system, which delivers 10 nm-wide illumination bands within the blue-green range, was developed.

The photobiology platform has also set up in-vitro cellular models of age-related macular degeneration (AMD) and glaucoma disorders based on primary retinal pigment epithelial cells (RPE) and retinal ganglion cells (RGC) cultures.

Our future projects include the development of new cellular models to study other retinopathies.


Action fields

  • Age-related macular degeneration (AMD)
  • Glaucoma


Equipments

The photobiology platform is equipped with laminar flow hoods and CO2 incubators in order to perform retinal primary cell cultures as well as retinal pigment epithelium (RPE) cells or ganglion cells.

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The photobiology platform has developed a cellular illumination system, which delivers 10 nm-wide illumination bands within the blue-green range (centered from390 to 520 nm in 10 nm increments, one additional band centered at 630 nm). Light irradiances are normalized with respect to the natural sunlight reaching the retina after being filtered by the ocular structures. Cells are illuminated in 96-well plates in order to define precisely the most phototoxic wavelengths of the visible light spectrum.

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Visible light spectrum delivered by the illumination system.


Team

serge

Serge Picaud

melanie

Mélanie Marie


Serge Picaud
Scientific supervisor

Email: Serge.Picaud@inserm.fr


Mélanie Marie
Technical manager

PhD in cellular and molecular biology, engineer in biotechnologies (R&D).

Expertise domains: ophthalmology, dermatology, oncology, Alzheimer’s disease, photobiology (ionizing radiation, UV, visible light), stem cells, DNA damage, oxidative stress, retinal cell physiology, cellular models of pathologies, R&D.
 
Technical skills: primary cell cultures (retinal cells, skin stem cells), proteins (western blot, ELISA, immunohistochemistry), nucleic acids (DNA and RNA extraction, purification, qPCR, transcriptomic analysis), cell stress (cell death, DNA damage, comet assay, cell cycle analysis).

Email: melanie.marie@inserm.fr