DONATE MAINS

biochimie4

The biochemistry platform of the Institut de la Vision manages equipments dedicated to protein biochemistry. It is specialized in protein-protein interactions and provides advice for the implementation and optimization of protocols.

Equipments:

  • Fusion Fx7 (Vilber Lourmat): Chemiluminescence and fluorescent imaging system (acquisition and analysis of  Western blot membrane) ;
  • ChemiDoc XR+ (Bio-Rad): Stain free imaging (normalization tools in Western blot analysis) ;
  • Bioanalyzer 2100 (Agilent Technologies): quantification and quality assessment of DNA, RNA and proteins;
  • NanoDrop 2000 (Thermo Scientific): concentration and purity measurements of DNA, RNA and proteins.

Assistance and advice:

  • Demo and trainings for autonomous equipment operation ;
  • Assistance for protocol implementation and optimization (1D electrophoresis, immunoprecipitation, lipid raft isolation, binding assay).

Sample preparation:

Additional services are being implemented, in particular the analysis of protein-protein interactions using FRET or Proximity Ligation Assay.

For requests and quotes, please send an email to the technical manager.


Publications

A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors.
Averaimo S, Assali A, Ros O, Couvet S, Zagar Y, Genescu I, Rebsam A, Nicol X.
Nat Commun. 2016 Oct 3;7:12896. doi: 10.1038/ncomms12896.

Signaling switch of the axon guidance receptor Robo3 during vertebrate evolution.
Zelina P, Blockus H, Zagar Y, Péres A, Friocourt F, Wu Z, Rama N, Fouquet C, Hohenester E, Tessier-Lavigne M, Schweitzer J, Roest Crollius H, Chédotal A.
Neuron. 2014 Dec 17;84(6):1258-72. doi: 10.1016/j.neuron.2014.11.004. Epub 2014 Nov 26.


Team

xavier small

Xavier Nicol

yvrick

Yvrick Zagar


Xavier Nicol
Scientific supervisor

Email: xavier.nicol@inserm.fr


Yvrick Zagar
Technical manager
Expertises in protein-protein interaction, analysis of signalling pathways, 1D electrophoresis, protein purification.

Email: yvrick.zagar@inserm.fr